While I can call tExposure(exp), I’m left with the problem that user can change the exposure using the MM input field, or the it can be changed programmatically, and my spinner will have no idea it was changed. > So, I’d also like to be able to controt the exposure from a UI component, specifically a spinner control. Ideally, I would take advantage of Andor's spooling feature, but I I can't seem to find a good way to save it directly from the Beanshell Used mmc.startSequenceAcquisition() to record a frame transfer series, but How can I save files programmatically? I've
Alternatively, I have decent experience with C/C++, but Iĭon't know where to find example code and would prefer not to deal with theĪdded complexity unless there are drawbacks to Beanshell (e.g.Ģ. I've never used it before, but I looked through some of the example scriptsĪnd they look promising (simple to write/modify, fair amount ofįunctionality + a large number of example scripts available on the Does it sound reasonable to program entirely in the Beanshell language? Since I'm very new to Micromanager, I have several fairly basic questionsġ.
Series using RS-232 and occasionally pulse it on for a few frames using theįast TTL outputs, controlled by a PIC or an Arduino board).
Is easier for our localization software to handle the memory load) and toīe able to adjust the AOTF-channels (maybe adjust the UV level between Transfer series (maybe 20 series of 500 frames each sub-divided because it Ideally, I would like to collect multiple frame
Localization microscopy (ultimately want to synchronize two Andor iXonĬameras and an AA-AOTF). I am very new to Micromanager and trying to build a flexible tool for Micro-manager-general mailing Sam and David, Improve your efficiency, and focus on delivering more value-add servicesĭiscover what IT Professionals Know. Remotely access PCs and mobile devices and provide instant support LogMeIn Rescue: Anywhere, Anytime Remote support for IT. > Live window and select Cy5, change the exposure time in the Live window to > exposure times, let's say Cy5 and DAPI with 100 ms each. > If I have two channels in the Multi-D acquisition window with manually set
> I open Multi-D and set up the channel which takes the live setting for Normally, I start in Live and determine the exposure I want. > I am confused as to what you are describing, as I have not had that On Dec 19, 2012, at 3:20 PM, Sam Lord wrote: Hope this will work nicely and intuitively for everyone, otherwise, please let us know.
What may be a bit confusing is that the Main window will remember the exposure times associated with a configuration preset, but only for the current channelgroup (which is something that you set in the MDA window). I add an option (unchecked by default) to couple the exposure times in the Main and MDA windows. Great discussion! Very helpful to get your input. Subject: Re: Two suggestions/requests for MicroManager Skirball Institute of Biomolecular Medicineįrom: Nico Stuurman Sunday, Decem9:05 PM Michael Cammer, Assistant Research Scientist Thank you! We'll try within the next week or so.